Kanghui-Bio has built a mature prokaryotic and eukaryotic high-throughput expression platform, based on the perfect E. coli culture system, yeast high-density fermentation system and HEK293 mammalian cell transient transfection system, to select the best expression route for different proteins, and to find the optimal balance between the expression cost, the amount of expression and the expression activity.

High-throughput expression platform based on E.coli

Escherichia coli (E. coli) was the first host used for recombinant expression. Prokaryotic expression systems are low-cost, high-efficiency, clear genetic background, and easy to scale up, making them important for both laboratory and industrial-scale production.

Kanghui-Bio provides one-stop service from codon optimization to recombinant protein expression/purification, and additional services such as endotoxin removal are available to meet customized needs. The laboratory staff has rich experience in soluble protein expression purification and inclusion body denaturation. The laboratory keeps a variety of commonly used vectors and host bacteria, which can meet the recombinant expression of different types of proteins and provide customers with high quality recombinant proteins.

High Density Fermentation Platform based on Yeast

Yeast cells combine the advantages of both prokaryotic and eukaryotic expression systems. While retaining low cost and high efficiency, they can also obtain proteins with post-translational modifications that are infinitely closer to their natural forms.

Kanghui-Bio can provide yeast fermentation expression at pilot level for rapid scale-up of recombinant proteins.

Transient Transfection Platform based on Mammalian Cells

Mammalian cells are an important component of modern recombinant protein biotechnology. As mammalian cells have highly complex mechanisms of protein folding and post-translational modification, recombinant proteins expressed through them possess the characteristics of correct folding and modification, as well as the closest natural form in vivo.

Kanghui-Bio uses HEK293T (Human Embryonic Kidney Cells) as the expression host for culture, which enables the expression of recombinant proteins and recombinant antibodies in high throughput and high yield. The transient expression technology of mammalian cells can be applied in the fields of antigen preparation, antibody discovery, protein structure and function research.